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Bioelectrics

 

Scientific focus in Bioelectrics is the interaction of pulsed electric fields (PEF) with biological cells, tissue and organisms. In general, for PEF exposure, the biological material is suspended in aqueous solution between two electrodes connected to a high voltage pulse generator. Depending on the pulse parameters, various effects and applications can be obtained: more...

Nanosecond time-resolved measurement of membrane charging

Membrane charging is a necessary condition for subsequent membrane permeabilization. The time constant of membrane charging is on the order of 100 ns. Thus, measurements on the dynamics of membrane charging demand for fast diagnostic tools like pulsed laser fluorescence microscopy (PLFM) exhibiting a temporal resolution of 5 ns. more...

Pulsed electric field treatment of microalgae

Microalgae are agreed to be a promising and sustainable future biomass feedstock. Economic recovery of intracellularly stored compounds is primary objective in current R&D efforts. The fractionating properties of pulsed electric field treatment open new perspectives for microalgae valorization. more...

Electrophysiological analysis of permeabilization processes

Commonly used electrode configurations in treatment chambers for PEF processing do not allow a direct measurement of the field-induced transmembrane voltage. This can be accomplished by applying the patch-clamp technique. more...

Plant stress responses due to ns pulse exposure

Electric field pulses with a short rise time and a duration in the sub-microsecond domain allow for penetration of the electric field inside the cell and for interaction with intracellular organelles. Among others, this can result in a growth stimulation of higher plants. more...

Treatment chambers for industrial-scale operation

Industrial applications of pulsed electric field treatment require for long-lifetime and failure-free operation of PEF treatment devices. Particular attention in scale-up developments at IHM is given to electrode effects encountered with single cell suspension treatment. more...